c1 manual  (fluidigm)

Journal: Nature

Article Title: Signalling by senescent melanocytes hyperactivates hair growth.

doi: 10.1038/s41586-023-06172-8

Figure Lengend Snippet: Fig. 2 | Hair SCs within nevus skin lose quiescence. a, On RNA-seq analysis, Tyr-NrasQ61K bulge SCs differ from P30 and P56 WT bulge SCs. A principal component analysis plot is shown. See Extended Data Fig. 6. b, A list of selected downregulated (red) and upregulated (green) genes at P56 and Tyr-NrasQ61K to WT fold change values. c, qRT–PCR of selected differentially expressed genes from a. n = 3. d, t-Distributed stochastic neighbour embedding (t-SNE) analysis on single-cell RNA-seq data for P30 and P56 WT and P56 Tyr-NrasQ61K bulge SCs. Cells form five clusters: C1 to C5. e, Cladogram showing relative cluster similarity. f, t-SNE plot colour-coded by sample source. g, t-SNE plot colour- coded by inferred cell cycle state. h, Violin plots for selected genes. See Extended Data Fig. 6. TPM, transcripts per million. i, EdU pulse-chase analysis on bulge SCs. Unlike total numbers of CD34+CD49f+ bulge SCs (top), their EdU+

Article Snippet: Single-cell RNA-seq libraries were constructed in 96-well plates according to the Fluidigm C1 manual.

Techniques: RNA Sequencing, Quantitative RT-PCR, Pulse Chase